Journal: bioRxiv

Article Title: A multi-pronged human microglia response to Alzheimer’s disease Aβ pathology

doi: 10.1101/2022.07.07.499139

Figure Lengend Snippet: Human cells were sorted according to the expression of CD11b+ hCD45+ whereas mouse cells were defined as CD11b+ hCD45-mCD45+. Note that we applied a trigger (=threshold) on CD11b+ events, therefore all CD11b negative cells are not displayed.

Article Snippet: Next, cells were washed in 5 ml of FACS buffer and pelleted cells were incubated with the following antibodies: PE-Pan-CD11b (1:50, Miltenyi, Cat#130-113-806), BV421-mCD45 (1:500, BD Biosciences, Cat#563890), APC-hCD45 (1:50, BD Biosciences, Cat#555485), TotalSeq™-A cell hashing antibodies (1:500, Biolegend) and viability dye (eFluor 780, Thermo Fisher Scientific, Cat#65-0865-14) in cold FACS buffer during 30 minutes at 4°C.

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Tumor cell-induced platelet aggregation accelerates hematogenous metastasis of malignant melanoma by triggering macrophage recruitment

doi: 10.1186/s13046-023-02856-1

Figure Lengend Snippet: TCIPA alters the phenotype and number of macrophages in the TIME. a The FCM protocol. b The proportion of F4/80 + CD11b + CD206 + cells among the live CD45 + cells in the fresh spleens ( e ), lung metastases ( f ) and primary tumors ( g ) from the B16 + PLT model, B16 model and control groups. * p ≤ 0.05, ** p ≤ 0.01, N = 3. The expression of F4/80 ( c ) and CD206 ( d ) in lung metastases was detected by IHC. The IOD values of both F4/80 ( h ) and CD206 ( i ) were significantly higher in the B16 + PLT model than in the B16 model. *** p ≤ 0.001, N = 15

Article Snippet: The RedFluorTM 710 Anti-Mouse CD45 (30-F-11) (TONBO Biosciences, catalog no. 80–0451, USA), PerCP-Cyanine5.5 anti-Mouse F4/80 antigen (BM8.1) (TONBO Biosciences, catalog no. 65–4801, USA), CD11b monoclonal antibody (M1/70) eFluor 450 (eBioscience, catalog no. 48–0112-82, USA), CD206 (MMR) monoclonal antibody (MR6F3) PE-Cyanine7 (eBioscience, catalog no. 25–2061-82, USA) were applied for staining according to the manufacturer's instructions.

Techniques: Control, Expressing

Journal: bioRxiv

Article Title: A multi-pronged human microglia response to Alzheimer’s disease Aβ pathology

doi: 10.1101/2022.07.07.499139

Figure Lengend Snippet: a , Experimental design used in this study. b , UMAP plot visualizing 127,755 single xenografted human microglial cells sorted from mouse brain (CD11b + hCD45 + ) ∼6 months after transplantation. Cells are coloured according to clusters identified: homeostatic (HM), Ribosomal response (RM), Disease Associated (DAM), Interferon response (IRM), Antigen presenting response (HLA), Cytokine response 1/2 (CRM-1 and CRM-2), and Transitioning CRM (tCRM). The assignment of different clusters to distinct cell types or states is based on previous experimental data from our and other laboratories , , , (see c, d and and ). c , UMAP plots as in (b), coloured by the combined level of expression of groups of genes that characterise distinct microglial transcriptional states. d , Top 10 most differentially expressed genes in each cluster (normalized expression scaled by gene is shown).

Article Snippet: Next, cells were washed in 5 ml of FACS buffer and pelleted cells were incubated with the following antibodies: PE-Pan-CD11b (1:50, Miltenyi, Cat#130-113-806), BV421-mCD45 (1:500, BD Biosciences, Cat#563890), APC-hCD45 (1:50, BD Biosciences, Cat#555485), TotalSeq™-A cell hashing antibodies (1:500, Biolegend) and viability dye (eFluor 780, Thermo Fisher Scientific, Cat#65-0865-14) in cold FACS buffer during 30 minutes at 4°C.

Techniques: Transplantation Assay, Expressing

Journal: bioRxiv

Article Title: A multi-pronged human microglia response to Alzheimer’s disease Aβ pathology

doi: 10.1101/2022.07.07.499139

Figure Lengend Snippet: Human cells were sorted according to the expression of CD11b+ hCD45+ whereas mouse cells were defined as CD11b+ hCD45-mCD45+. Note that we applied a trigger (=threshold) on CD11b+ events, therefore all CD11b negative cells are not displayed.

Article Snippet: Next, cells were washed in 5 ml of FACS buffer and pelleted cells were incubated with the following antibodies: PE-Pan-CD11b (1:50, Miltenyi, Cat#130-113-806), BV421-mCD45 (1:500, BD Biosciences, Cat#563890), APC-hCD45 (1:50, BD Biosciences, Cat#555485), TotalSeq™-A cell hashing antibodies (1:500, Biolegend) and viability dye (eFluor 780, Thermo Fisher Scientific, Cat#65-0865-14) in cold FACS buffer during 30 minutes at 4°C.

Techniques: Expressing